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Objective@#To compare the diagnosis and treatment experience of brain abscesses and improve prognosis.@*Methods@#The data of 302 patients of brain abscess at Department of Neurosurgery in Tianjin Medical University General Hospital from 1980 to 2014 was analyzed retrospectively. There were 215 male and 87 female patients aged from 11 to 82 years with mean age of (30±8) years. The patients was divided into 1980-2001 group and 2002-2014 group according to different diagnosis and the treatment methods. The therapy methods include operation and conservative treatment. There were 196 cases received operation, including 95 cases of excision, 89 cases of ventriculopuncture, 12 cases of excision after ventriculopuncture, 106 cases received drug conservative therapy. Two groups of information including clinical manifestation, abscess location, therapeutic effect and prognosis were compared by χ2 test.@*Results@#Compared to 1980-2001 group, adjacent infection incidence declined(χ2=8.000, P=0.005). The ratio of single abscess declined and multiple abscess increased(χ2=11.060, P=0.001), the infection proportion of frontal lobe and temporal lobe decreased(χ2=9.080, P=0.003; χ2=15.440, P=0.000). The ratio of headache and vomit and papilledema declined significantly(χ2=23.290, P=0.000; χ2=21.020, P=0.000; χ2=2.290, P=0.001). Total mortality of 302 patients were 23 cases and 5 cases of 1980-2001 group and 2002-2014 group (10.4% vs. 6.3%, χ2=1.180, P=0.277). However, there were statistical difference in postoperative mortality between both groups (14.4% vs. 4.0%, χ2 =3.880, P=0.049).@*Conclusion@#With the application of antibiotics and the development of neurosurgical techniques, the prognosis of brain abscess has been improved.
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Objective To observe the effect of laparoscopy combined with choledochoscopy in treatment of cholelithiasis clinical effect .Methods 80 cases diagnosed as cholelithiasis cases were divided into control group and observation group with 40 cases in each group .The control group used the traditional laparotomy incision gallbladder stone ;observation group were treated by laparos-copy combined with choledochoscopy .Results In the observation group ,the operation and the hospital stay were significantly shor-ter than the control group ,operation curative effect was obviously superior to the control group ,the incidence of postoperative com-plications was significantly lower than the control group .Conclusion The use of laparoscopy combined with choledochoscopy tech-nology in treatment of gallstones have good clinical effect .
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Objective To investigate the expression of C-JUN activation domain binding protein 1(JAB1)and its relationship with expression of P27 protein in human hepatocellular carcinoma(HCC),and to determine whether JAB1 is associated with clinicopathological parameters and prognosis of HCC.Methods Immunohistochemical analysis was performed to investigate the expression of JAB1 and P27 in 76 cases of HCC and adjacent nontumorous tissues.Fresh tumor tissues and their adjacent nontumorous tissues from 8 cases of HCC were collected for Western blot and immunoprecipitation assays.Results The expression of JAB1 in HCC was significantly higher than that in adjacent nontumorous tissues.In contrast,P27 level was higher in nontumorous liver tissues than that in HCC.JAB1 overexpression was correlated with histological differentiation,serum alpha-fetoprotein(AFP)level and metastasis(P
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Objective To investigate the relationship between growth inhibiting effect of arsenic trioxide(As_2O_3) and phosphorylation of P27kip threonine residue 187(P27T187) in human hepatocellular carcinoma(HCC) cell line SMMC-7721.Methods SMMC-7721 were treated for 72 h with 2 ?mol/L As_2O_3.The cell growth inhibition was detected by cell counting and the cell cycle was detected by flow cytometry(FCM).The expression and localization of P27,T187 phosphorylated P27(p-P27T187) were detected by Subcellular Fractionation,Western blot and immunoflurescence.Results As_2O_3 significantly inhibited the proliferation of SMMC-7721 cell and cell cycle was arrested in G2/M.A significant decrease in p-P27T187 expression and a reciprocal increase in P27 expression were found in 2 ?mol/L As_2O_3-treated SMMC-7721 cell.Meanwhile,As_2O_3 decreased the protein levels of Cdk2 and cyclinE.The location of P27 was transferred from cytoplasm to nuclei and the expression of p-P27T187 was decreased in nuclei.Conclusion As_2O_3 inhibits the phosphorylation of P27T187,thereby promoting P27 accumu-lation in SMMC-7721 cell nuclei,inducing cel1 cycle arrest and growth inhibition.
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Objective To explore the correlation between carotid atherosclerosis and cerebral infarction through checking carotid atherosclerosis.Methods Intima-media thickness of carotid arterial,carotid atherosclerosis plaque score and degree of stenosis are measured in both infarction groups(200 cases)and control group(200 cases).Patients with carotid atherosclerosis plaque were treated with aspirin and siavaastatin and then the plaque after theray was compared with itself before theray in size of plaque and degree of sclerosis.Resluts The incidence rate of carotid atherosclerosis plaque is 70% in infarction group and 28% in control group;The incidence rate of moderate-severe carotid stenosis is 22% in infarction group and 6% in control group.The difference between infarction and control group is significant in intima-media thickness of carotid arterial,size of carotid atherosclerosis plaque and level of stenosis(P